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input whole genome bisulfite sequencing  (Novogene)

 
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    Structured Review

    Novogene input whole genome bisulfite sequencing
    <t>Whole-genome</t> <t>DNA</t> methylation analysis of Chinese yam variant F2000 and F60. A Principal component 1 and principal component 2 analyses were performed for each sample. B Methylation level distribution across genetic features including 4 kb upstream of TSS, gene body, and 4 kb downstream of TTS in the three DNA methylation contexts CG, CHG, and CHH. Analyses were performed in biological triplicates for each variant. C Number of DMRs with higher methylation levels in F2000 (hypermethylated) and in F60 (hypomethylated). DMRs were investigated in the three DNA methylation contexts CG, CHG and CHH. Distribution of DMRs upstream in the putative promoter (4 kb of TSS), within the gene body, and down-stream in the putative terminator (4 kb of TTS) were analyzed in DMR-associated genes (q-value ≤ 0.01, methylation difference >|10%|). D Venn analysis of DMR-associated genes split in hyper- and hypomethylated promoters, gene bodies, and terminators. DMR, differentially methylated region; TSS, transcription start site; TTS, transcription termination site
    Input Whole Genome Bisulfite Sequencing, supplied by Novogene, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/input whole genome bisulfite sequencing/product/Novogene
    Average 86 stars, based on 1 article reviews
    input whole genome bisulfite sequencing - by Bioz Stars, 2026-06
    86/100 stars

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    1) Product Images from "Insights into epigenetics suggest a role of DNA methylation in regulating Chinese yam tuber shape"

    Article Title: Insights into epigenetics suggest a role of DNA methylation in regulating Chinese yam tuber shape

    Journal: BMC Plant Biology

    doi: 10.1186/s12870-026-08438-5

    Whole-genome DNA methylation analysis of Chinese yam variant F2000 and F60. A Principal component 1 and principal component 2 analyses were performed for each sample. B Methylation level distribution across genetic features including 4 kb upstream of TSS, gene body, and 4 kb downstream of TTS in the three DNA methylation contexts CG, CHG, and CHH. Analyses were performed in biological triplicates for each variant. C Number of DMRs with higher methylation levels in F2000 (hypermethylated) and in F60 (hypomethylated). DMRs were investigated in the three DNA methylation contexts CG, CHG and CHH. Distribution of DMRs upstream in the putative promoter (4 kb of TSS), within the gene body, and down-stream in the putative terminator (4 kb of TTS) were analyzed in DMR-associated genes (q-value ≤ 0.01, methylation difference >|10%|). D Venn analysis of DMR-associated genes split in hyper- and hypomethylated promoters, gene bodies, and terminators. DMR, differentially methylated region; TSS, transcription start site; TTS, transcription termination site
    Figure Legend Snippet: Whole-genome DNA methylation analysis of Chinese yam variant F2000 and F60. A Principal component 1 and principal component 2 analyses were performed for each sample. B Methylation level distribution across genetic features including 4 kb upstream of TSS, gene body, and 4 kb downstream of TTS in the three DNA methylation contexts CG, CHG, and CHH. Analyses were performed in biological triplicates for each variant. C Number of DMRs with higher methylation levels in F2000 (hypermethylated) and in F60 (hypomethylated). DMRs were investigated in the three DNA methylation contexts CG, CHG and CHH. Distribution of DMRs upstream in the putative promoter (4 kb of TSS), within the gene body, and down-stream in the putative terminator (4 kb of TTS) were analyzed in DMR-associated genes (q-value ≤ 0.01, methylation difference >|10%|). D Venn analysis of DMR-associated genes split in hyper- and hypomethylated promoters, gene bodies, and terminators. DMR, differentially methylated region; TSS, transcription start site; TTS, transcription termination site

    Techniques Used: DNA Methylation Assay, Variant Assay, Methylation



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    Novogene input whole genome bisulfite sequencing
    <t>Whole-genome</t> <t>DNA</t> methylation analysis of Chinese yam variant F2000 and F60. A Principal component 1 and principal component 2 analyses were performed for each sample. B Methylation level distribution across genetic features including 4 kb upstream of TSS, gene body, and 4 kb downstream of TTS in the three DNA methylation contexts CG, CHG, and CHH. Analyses were performed in biological triplicates for each variant. C Number of DMRs with higher methylation levels in F2000 (hypermethylated) and in F60 (hypomethylated). DMRs were investigated in the three DNA methylation contexts CG, CHG and CHH. Distribution of DMRs upstream in the putative promoter (4 kb of TSS), within the gene body, and down-stream in the putative terminator (4 kb of TTS) were analyzed in DMR-associated genes (q-value ≤ 0.01, methylation difference >|10%|). D Venn analysis of DMR-associated genes split in hyper- and hypomethylated promoters, gene bodies, and terminators. DMR, differentially methylated region; TSS, transcription start site; TTS, transcription termination site
    Input Whole Genome Bisulfite Sequencing, supplied by Novogene, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    low input whole genome bisulfite sequencing prepared dna  (Novogene)
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    <t>Whole-genome</t> <t>DNA</t> methylation analysis of Chinese yam variant F2000 and F60. A Principal component 1 and principal component 2 analyses were performed for each sample. B Methylation level distribution across genetic features including 4 kb upstream of TSS, gene body, and 4 kb downstream of TTS in the three DNA methylation contexts CG, CHG, and CHH. Analyses were performed in biological triplicates for each variant. C Number of DMRs with higher methylation levels in F2000 (hypermethylated) and in F60 (hypomethylated). DMRs were investigated in the three DNA methylation contexts CG, CHG and CHH. Distribution of DMRs upstream in the putative promoter (4 kb of TSS), within the gene body, and down-stream in the putative terminator (4 kb of TTS) were analyzed in DMR-associated genes (q-value ≤ 0.01, methylation difference >|10%|). D Venn analysis of DMR-associated genes split in hyper- and hypomethylated promoters, gene bodies, and terminators. DMR, differentially methylated region; TSS, transcription start site; TTS, transcription termination site
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    Image Search Results


    Whole-genome DNA methylation analysis of Chinese yam variant F2000 and F60. A Principal component 1 and principal component 2 analyses were performed for each sample. B Methylation level distribution across genetic features including 4 kb upstream of TSS, gene body, and 4 kb downstream of TTS in the three DNA methylation contexts CG, CHG, and CHH. Analyses were performed in biological triplicates for each variant. C Number of DMRs with higher methylation levels in F2000 (hypermethylated) and in F60 (hypomethylated). DMRs were investigated in the three DNA methylation contexts CG, CHG and CHH. Distribution of DMRs upstream in the putative promoter (4 kb of TSS), within the gene body, and down-stream in the putative terminator (4 kb of TTS) were analyzed in DMR-associated genes (q-value ≤ 0.01, methylation difference >|10%|). D Venn analysis of DMR-associated genes split in hyper- and hypomethylated promoters, gene bodies, and terminators. DMR, differentially methylated region; TSS, transcription start site; TTS, transcription termination site

    Journal: BMC Plant Biology

    Article Title: Insights into epigenetics suggest a role of DNA methylation in regulating Chinese yam tuber shape

    doi: 10.1186/s12870-026-08438-5

    Figure Lengend Snippet: Whole-genome DNA methylation analysis of Chinese yam variant F2000 and F60. A Principal component 1 and principal component 2 analyses were performed for each sample. B Methylation level distribution across genetic features including 4 kb upstream of TSS, gene body, and 4 kb downstream of TTS in the three DNA methylation contexts CG, CHG, and CHH. Analyses were performed in biological triplicates for each variant. C Number of DMRs with higher methylation levels in F2000 (hypermethylated) and in F60 (hypomethylated). DMRs were investigated in the three DNA methylation contexts CG, CHG and CHH. Distribution of DMRs upstream in the putative promoter (4 kb of TSS), within the gene body, and down-stream in the putative terminator (4 kb of TTS) were analyzed in DMR-associated genes (q-value ≤ 0.01, methylation difference >|10%|). D Venn analysis of DMR-associated genes split in hyper- and hypomethylated promoters, gene bodies, and terminators. DMR, differentially methylated region; TSS, transcription start site; TTS, transcription termination site

    Article Snippet: Prepared DNA samples were sent to Novogene Company (Beijing, China) for low-input whole-genome bisulfite sequencing including the steps of bisulfite treatment and library construction.

    Techniques: DNA Methylation Assay, Variant Assay, Methylation